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ProteomicsProteomic analysis of integrin activation-state-dependent adhesion complexes
Proteomic analysis of integrin activation-state-dependent adhesion complexes. Adhesion complexes were isolated from K562 cells using activation-state-specific monoclonal antibodies coupled to magnetic beads.Tandem mass spectra were extracted using extract_msn (Thermo Fisher Scientific) executed in M...
ORGANISM(S): Homo sapiens (Human) 
2015-01-26 | PXD000155 | Pride
Human K562 Adhesion complex Integrin Activation state Ms Proteomics 
ProteomicsProteomic analysis of alpha4beta1 integrin adhesion complexes reveals alpha-subunit-dependent protein recruitment
Peptides and proteins identified for VCAM-1(D40A) control ligand affinity purification using K562-alpha4 cells (biological replicate 1)
ORGANISM(S): Homo sapiens (Human) 
2012-05-27 | PRD000565 | Pride
ProteomicsProteomic analysis of ECM supporting hESC maintenance
Comparative analysis of extracellular matrix proteins in substrates supportive and unsupportive for human embryonic stem cell maintenance
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2013-05-30 | PRD000600 | Pride
ProteomicsCharacterisation of FAK- and ILK-dependent nuclear subproteomes
In addition to central functions in cell adhesion signalling, integrin-associated proteins have wider roles at sites distal to adhesion receptors. In experimentally defined adhesomes, there is clear enrichment of proteins that localise to the nucleus, and conversely, we now report that nuclear prote...
ORGANISM(S): Mus musculus (Mouse) 
2022-06-09 | PXD020179 | Pride
Cancer Ilk Adhesion Nucleus Human Fak 
ProteomicsProteomic analysis of IL33-proximal proteins in squamous cell carcinoma cells
Focal adhesion kinase (FAK) is required for the expression of pro-inflammatory genes that inhibit anti-tumour immunity. Here, we show a novel and crucial role for the dual-function cytokine IL33 in FAK-dependent immune evasion. Specifically, nuclear FAK is required for the expression of IL33, the ch...
ORGANISM(S): Mus musculus (Mouse) 
2017-12-06 | PXD007698 | Pride
Fak Transcription Proteomics Tumour growth Lc-ms Il33 Il1rl1 Scc Bioid St2 Chromatin 
ProteomicsAmbra1 spatially regulates Src activity and Src/FAK-mediated cancer cell invasion via trafficking networks
Here, using mouse squamous cell carcinoma cells, we report a completely new function for the autophagy protein Ambra1 as the first described ‘spatial rheostat’ controlling the Src/FAK pathway. Ambra1 regulates the targeting of active phospho-Src away from focal adhesions into autophagic structures t...
ORGANISM(S): Mus musculus (Mouse) 
2017-04-04 | PXD006002 | Pride
Ambra1 Ifitm3 Trafficking Invasion Src Fak Dynactin 1 
ProteomicsProteomic analysis of Raptor-proximal proteins in U2OS cells
The mammalian target of rapamycin complex 1 (mTORC1) is a key nutrient-sensing hub and a master regulator of cellular growth and metabolism. Activation of mTORC1 by amino acids and growth factors requires its recruitment to the lysosomes. Furthermore, lysosomal distribution has been shown to be inti...
ORGANISM(S): Homo sapiens (Human) 
2021-09-08 | PXD012795 | Pride
Mtorc1 Raptor Ms Q exactive plus Proximity labelling Proximity-based biotinylation Human Bioid Bioid2 Osteosarcoma Lc-ms/ms 
TranscriptomicsPulmonary gene and microRNA expression changes in mice exposed to benzo(a)pyrene by oral gavage
Polycyclic aromatic hydrocarbons (PAHs) are widely distributed, carcinogenic environmental contaminants produced as a consequence of the incomplete combustion of fossil fuels. Benzo[a]pyrene (BaP) is a very extensively studied prototypical PAH. We recently reported a complete lack of change in micro...
ORGANISM(S): Mus musculus 
2011-10-03 | E-GEOD-24751 | biostudies-arrayexpress
ProteomicsCharacterisation of the adhesome of patient-derived cutaneous squamous carcinoma cells
Interactions between cells and the extracellular matrix, mediated by integrin adhesion complexes (IACs), play key roles in cancer progression and metastasis. We report here systems-level changes in the adhesome during progression of a patient-derived cutaneous squamous cell carcinoma (cSCC). We foun...
ORGANISM(S): Homo sapiens (Human) 
2023-03-25 | PXD021492 | Pride
Cancer Adhesome Adhesion Human 
ProteomicsCharacterisation of a nucleo-adhesome
In addition to central functions in cell adhesion signalling, integrin-associated proteins have wider roles at sites distal to adhesion receptors. In experimentally defined adhesomes, we noticed that there is clear enrichment of proteins that localise to the nucleus, and conversely, we now report th...
ORGANISM(S): Mus musculus (Mouse) 
2022-06-01 | PXD025870 | Pride
Adhesome Adhesion Nucleus Human 
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