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ProteomicsPhosphoproteome of five-minute KCl-depolarised mouse cultured hippocampal neurons
Cultured hippocampal neurons from mice were treated with high concentration KCl for five minutes to depolarise the plasma membrane and model sustained brain activity. A high KCl condition was compared to low KCl using phosphoproteomics. Phosphopeptides were enriched from trypsin digested bio-replica...
ORGANISM(S): Mus musculus (Mouse) 
2023-10-15 | PXD020232 | Pride
Neurons Depolarisation Phosphoproteome Mouse Hippocampal Tau Kcl Lc-ms/ms 
ProteomicsProteomics characterisation of induced pluripotent stem cells and cortical brain organoids.
A method was developed to reproducibly produce neural retina and cortical brain regions from confluent cultures of stem cells. The spontaneously generated cortical organoids were isolated and cultured in suspension conditions for maturation. Proteomic analysis of both the original induced pluripoten...
ORGANISM(S): Homo sapiens (Human) 
2022-05-06 | PXD025933 | Pride
Cortical Stem cell Proteomics Brain Neural retina Organoid Pluripotent 
ProteomicsPRM validation of phosphoproteomic and proteomic analysis of early epileptogenesis in mice at 4 and 24 h post-status epilepticus
The phosphorylation-based signalling and protein changes occurring in the early phases after a pathophysiological insult, like status epilepticus (SE), have not been detailed. In a companion project, the hippocampi of mice treated with pilocarpine and diazepam were examined by tandem mass tag (TMT11...
ORGANISM(S): Mus musculus (Mouse) 
2024-02-22 | PXD047870 | Pride
Epileptogenesis Phospho-signalling Proteomics Epilepsy Hippocampus Synaptic weakening Signalling Phosphorylation Mouse Parallel reaction monitoring Synapse growth 
ProteomicsPhosphoproteomic and proteomic analysis of early epileptogenesis in mice at 4 and 24 h post-status epilepticus
The phosphorylation-based signalling and protein changes occurring in the early phases after a pathophysiological insult, like status epilepticus, have not been detailed. To this end, the hippocampi of mice treated with pilocarpine and diazepam were examined by mass spectrometry at 4 and 24 h post-s...
ORGANISM(S): Mus musculus (Mouse) 
2024-02-22 | PXD038241 | Pride
Epileptogenesis Phosphoproteome Epilepsy Hippocampus Phosphorylation Mouse Proteome Tandem mass tag Phospho-signalling 
ProteomicsThe temporal profile of activity-dependent presynaptic phospho-signalling reveals long lasting patterns of post-stimulus regulation
Depolarization of presynaptic terminals stimulates calcium influx, which evokes neurotransmitter release and activates phosphorylation-based signalling. Here, we present the first global temporal profile of presynaptic activity-dependent phospho-signalling, which includes two KCl stimulation levels ...
ORGANISM(S): Rattus norvegicus (Rat) 
2019-01-28 | PXD010007 | Pride
Neuron Phosphorylation Rat Phosphatase Signalling Synaptosome Presynaptic Depolarization Kinase Post-stimulus Pp1 
ProteomicsPhosphoproteomic analysis of mouse neurons with SRPK2 overexpression or knockdown
SRPK2 was identified as a presynaptic protein kinase involved in the regulation of neurotransmitter release. The phosphoproteome of mouse primary neurons was screened, under the conditions of SRPK2 overexpression and SRPK2 knockdown, to identify candidate substrates of SRPK2.
ORGANISM(S): Mus musculus (Mouse) 
2022-02-14 | PXD016636 | Pride
Brain Mouse Phosphorylation Srpk2 
TranscriptomicsHistologically normal epithelium from breast cancer patients and cancer-free prophylactic mastectomy patients
Gene expression in histologically normal epithelium from breast cancer patients and cancer-free prophylactic mastectomy patients share a similar profile Introduction: We hypothesized that gene expression in histologically normal epithelium (NlEpi) would differ in breast cancer patients (HN) compared...
ORGANISM(S): Homo sapiens 
2010-06-08 | E-GEOD-20437 | biostudies-arrayexpress
MetabolomicsMetabolic differences in ripening of Solanum lycopersicum 'Ailsa Craig' and three monogenic mutants (alternative assay to MTBLS36)
The purpose of this study was to highlight metabolic differences between different cultivars of tomato through development and ripening. Application of mass spectrometry enables the detection of metabolic differences between groups of related organisms. Differences in the metabolic fingerprints of w...
2017-10-18 | MTBLS73 | MetaboLights
MetabolomicsMetabolic differences in ripening of Solanum lycopersicum 'Ailsa Craig' and three monogenic mutants
Application of mass spectrometry enables the detection of metabolic differences between groups of related organisms. Differences in the metabolic fingerprints of wild-type Solanum lycopersicum and three monogenic mutants, ripening inhibitor (rin), non-ripening (nor) and Colourless non-ripening (Cnr)...
2014-02-07 | MTBLS36 | MetaboLights
ProteomicsKetogenic diet modifies ribosomal protein dysregulation in KMT2D Kabuki Syndrome
Kabuki syndrome (KS) is a genetic disorder caused by DNA mutations in KMT2D, a lysine methyltransferase that methylates histones and other proteins, and therefore modifies chromatin structure and subsequent gene expression. Ketones, derived from the ketogenic diet, are histone deacetylase inhibitors...
ORGANISM(S): Homo sapiens (Human) 
2024-06-23 | PXD050547 | Pride
Neurodevelopment Tmtpro Butyrate Hydrophilic interaction liquid chromatography Single-cell rna sequencing Lysine methyltransferase Epigenetic Tandem mass tag 
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