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ProteomicsComparative analysis of MS sample preparation methods
We compare the most commonly used proteomic sample preparation strategies such as classical in solution digest protocols, SPEED, FASP, STrap, SP3, as well as the commercially available kits iST (PreOmics) and EasyPep (Thermo Scientific) in their efficacy to extract proteomes from HeLa cells. We find...
ORGANISM(S): Homo sapiens (Human) 
2022-09-06 | PXD030406 | Pride
Hela Human Sample preparation Lc-ms/ms 
ProteomicsDeletion of GSE1 and HDAC1 inactivation alters proteomic profiles and cell cycle control
The DNA damage response (DDR) is orchestrated by multiple, tightly regulated signalling events. We discovered that genetic suppressor element 1 (GSE1) forms a complex with the histone deacetylation 1 (HDAC1) / CoREST complex and that loss of GSE1 hampers DDR induction. To investigate how GSE1 and HD...
ORGANISM(S): Homo sapiens (Human) 
2023-10-23 | PXD040339 | Pride
Acetylome Hdac1 Gse1 Phosphorylome Hap1 Dna damage response 
ProteomicsTMT Multiplexing-Based Quantitative Analysis of Pathogenic Th2 Proteomes.
To investigate the regulation of pathogenic Th2 (pTh2) cells and the role of histone deacetylase 1 (HDAC1) in the respective processes, we used a new in vitro protocol for generating pTh2 cells and performed qunatitative mass spectrometry analysis based on TMTpro 16plex multiplexing to analyze prote...
ORGANISM(S): Mus musculus (Mouse) 
2025-02-20 | PXD042975 | Pride
Tmtpro 16plex Pathogenic th2 cells Mus musculus Th2 cells 
ProteomicsNovel interconnections of HOG signaling revealed by combined use of two proteomic software packages
Modern quantitative mass spectrometry (MS)-based proteomics enables researchers to unravel signaling networks by monitoring proteome-wide cellular responses to different stimuli. MS-based analysis of signaling systems usually requires an integration of multiple quantitative MS experiments, which rem...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-06-11 | PXD011935 | Pride
Orm2 Hog1 P38 Mitogen-activated protein kinase (mapk) Hyperosmotic stress response Ormdl Maxquant High-osmolarity glycerol (hog) Kic1 Proteomics Proteome discoverer 
ProteomicsNovel interconnections of HOG signaling revealed by combined use of two proteomic software packages
Modern quantitative mass spectrometry (MS)-based proteomics enables researchers to unravel signaling networks by monitoring proteome-wide cellular responses to different stimuli. MS-based analysis of signaling systems usually requires an integration of multiple quantitative MS experiments, which rem...
ORGANISM(S): Saccharomyces Cerevisiae 
PXD013789 | panorama
ProteomicsAutophagy preferentially degrades non-fibrillar polyQ aggregates
Aggregation of proteins containing expanded polyglutamine (polyQ) repeats is the cytopathologic hallmark of a group of dominantly inherited neurodegenerative diseases, including Huntington's disease (HD). Huntingtin (Htt), the disease protein of HD, forms amyloid-like fibrils by liquid-to-solid phas...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2026-10-23 | PXD044261 | Pride
Autophagosome Human hek293 Mouse neuro2a Poly-glutamine Huntingtin 
ProteomicsEndosulfines Igo1 and Igo2 promote hyperosmotic stress signaling via inhibition of protein phosphatase 2A
The hyperosmotic stress response in budding yeast is a paradigm for cellular responses to physicochemical stimuli that is often used for modeling signal transduction pathways. Here, we describe the phosphatase PP2A-Cdc55 as a novel master regulator of hyperosmotic stress signaling. We show that its ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-11-02 | PXD019646 | Pride
Stress response Phosphorylation Igo2 Gis1 Igo1 Phosphatase Pp2a Saccharomyces cerevisiae Quantitative proteomics Rph1 Hyperosmotic stress Stress signaling Cdc55 Endosulfines 
ProteomicsPeroxiredoxin promotes longevity and H2O2-resistance in yeast through redox-modulation of protein kinase A
Peroxiredoxins are modulators of aging in yeast and multicellular organisms. The mechanisms by which peroxiredoxins stimulate H2O2 resistance and slow down aging are, however, unclear. Here we show that the yeast peroxiredoxin Tsa1 boosts H2O2 resistance and prevents aging independent of cellular H2...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-07-27 | PXD012617 | Pride
Prm Yeast Oxidative stress Saccharomyces cerevisiae Lc-ms/ms 
MetabolomicsGNPS - Dataset Creation from GNPS Molcular Networking - 89895d4507c84596a6a01685bfd8acd4
Molecular Networking of fungal culture extracts from T. erinacei analyzed by LC-MS in positive ion mode
ORGANISM(S): Trichophyton Erinacei (ncbitaxon:523106) 
2021-04-29 | MSV000087335 | GNPS
Genomicsena-DATASET-GoT2D/T2D-GENES-08-07-2016-21:22:44:693-1130 - samples
The T2D-GENES/GoT2D 13K exome sequencing study includes ~13,000 samples, half T2D cases and half T2D controls, from five ancestries (~5K Europeans, ~2K each of African-American, East-Asian, South-Asian, and Hispanic). Samples underwent deep exome sequencing, with SNVs and INDEls called according to ...
EGAD00001002246 | EGA
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