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ProteomicsProtein content and proximity map of yeast nuclear mRNPs
We isolated nuclear mRNPs from yeast using an endogenous bi-molecular tagging approach on Sub2 and Hpr1.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-07-25 | PXD040736 | Pride
Cross-linking mass spectrometry (xl-ms) Mrna synthesis Mrnp assembly Rna annealing Ribonucleoprotein particles 
ProteomicsThe EMC acts as a chaperone to labile transmembrane domains
Structure formation of membrane proteins is error-prone and thus requires chaperones that oversee this essential process in cell biology. The ER membrane protein complex (EMC) is well-defined as a transmembrane domain (TMD) integrase. In this study, we characterize an additional chaperone function o...
ORGANISM(S): Homo sapiens (Human) 
2025-08-25 | PXD059792 | Pride
Membrane proteins Endoplasmic reticulum Chaperone Protein folding 
ProteomicsCullin-RING ligases target substrates with geometrically optimized catalytic partners
Cullin-RING ubiquitin ligases (CRLs) control the degradation of a wide landscape of human proteins in combination with ubiquitin-carrying enzymes (UCEs). CRL expansion during evolution is apparent, with a few dozen in yeast that function with a single UCE and as many as 300 in humans that function w...
ORGANISM(S): Homo sapiens (Human) 
2024-05-22 | PXD043523 | Pride
Ubiquitylation Cullin-ring ligase 
ProteomicsNse5/6 inhibits the Smc5/6 ATPase and modulates DNA substrate binding
Eukaryotic cells employ three SMC complexes to control DNA folding and topology. The Smc5/6 complex plays roles in DNA repair and in preventing the accumulation of deleterious DNA junctions. To elucidate how specific features of Smc5/6 govern these functions, we reconstituted the yeast holo-complex....
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-07-02 | PXD024160 | Pride
Atpase Condensin Loop extrusion Chromosome segregation Xl-ms Smc5 Smc Smc6 Genome maintenance Cohesin 
ProteomicsSpo13/MEIKIN ensures a two-division meiosis by preventing the activation of APC/CAma1 at meiosis I
Genome haploidization at meiosis depends on two consecutive nuclear divisions, which are controlled by an oscillatory system consisting of Cdk1-cyclin B and the APC/C bound to the Cdc20 activator. How the oscillator generates exactly two divisions has been unclear. We have studied this question in y...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-09-03 | PXD044597 | Pride
Apc/c-ama1 / cyclin phosphorylation / meiotic exit / polo-like kinase / spo13/meikin / translational repressor 
ProteomicsAP-MS of replisomes from S. cerevisiae in S phase and after unscheduled G1 replication
Interfering with once-per-cell-cycle regulation of DNA replication initiation generates genome instability through over-replication and has been linked to early stages of cancer development. Here, we engineered different systems in budding yeast to induce over-replication in G1 and investigated its ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-03-11 | PXD035629 | Pride
Dna replication Gins complex 
ProteomicsDoa10/MARCH6 architecture interconnects E3 ligase activity with lipid-binding transmembrane channel to regulate SQLE
Transmembrane E3 ligases play crucial roles in homeostasis. Much protein and organelle quality control, and metabolic regulation, are determined by ER-resident MARCH6 E3 ligases, including Doa10 in yeast. Here, we present Doa10/MARCH6 structural analysis by cryo-EM and AlphaFold predictions, and a s...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD047499 | Pride
Ubiquitylation E3 ubiquitin ligases March6 Membrane proteins Doa10 Cryo-em Sqle 
ProteomicsCryo-EM structures of Gid12-bound GID E3 reveal steric blockade as a mechanism inhibiting substrate ubiquitylation
Protein degradation, a major eukaryotic response to cellular signals, is subject to numerous layers of regulation. In yeast, the evolutionarily conserved GID E3 ligase mediates glucose-induced degradation of fructose-1,6-bisphosphatase (Fbp1) and other gluconeogenic enzymes. “GID” is a collection of...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-05-29 | PXD028579 | Pride
Gid E3 ligase complex Crzoem 
Proteomicsbeta 1 integrin signaling governs necroptosis via the chromatin remodeling factor CHD4
Fibrosis, characterized by sustained activation of myofibroblasts and excessive extracellular matrix (ECM) deposition, is known to be associated with chronic inflammation. RIPK3, a key kinase mediating TNF-driven necroptosis signaling, is upregulated in fibrosis and contributes to the TNF-mediated i...
ORGANISM(S): Mus musculus (Mouse) 
2023-10-30 | PXD041505 | Pride
Ripk3 Necroptosis Integrin Inflammation Chd4 Fibrosis 
ProteomicsA SAM key domain required for enzymatic activity and allosteric activation of the Fun30 nucleosome remodeler
Fun30 is the prototype of the Fun30-SMARCAD1-ETL sub-family of nucleosome remodelers involved in DNA repair and gene silencing. These proteins appear to act as single subunit nucleosome remodelers, but their molecular mechanisms are, at this point, poorly understood. Using multiple sequence alignmen...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-07-18 | PXD037249 | Pride
Sam Fun30 Yeast Chromatin Resection Regulation Key 
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