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ProteomicsPAXX and its paralogues synergistically direct DNA polymerase I activity in DNA repair
In this study, we characterised the interactomes of PAXX and its paralogs, XLF and XRCC4, to show that these factors share the ability to interact with DNA polymerase lambda (Poll), stimulate its activityand are required for the recruitment of Poll to laser-induced DNA damage sites.
ORGANISM(S): Homo sapiens (Human) 
2018-09-24 | PXD010891 | Pride
Polymerase lambda Xrcc4 Double strand breaks Nhej Paxx Dna repair Xlf Dna-pkcs 
ProteomicsIdentifying mTOR regulated RNA binding proteins
To investigate the RNA binding proteins that change following mTOR inhibition, HeLa cells were treated with and without 200nM Torin1 for an hour. Cells were the UV254 cross-linked, lysed and large scale whole cell oligo(dT) based pulldowns were performed with identification and quantification of pu...
ORGANISM(S): Homo sapiens (Human) 
2020-11-24 | PXD016705 | Pride
Nanoflow. Larp1 Mtor Synapt g2s Rna-binding proteins 
ProteomicsA heme-binding domain in a voltage gated porassium channel
Heme-affinity pull-down assays and proteomics of lysates from primary cortical neurons identified and EAG channel, hERG3 (Kv11.3) binds to heme.
ORGANISM(S): Mus musculus (Mouse) 
2020-09-17 | PXD019887 | Pride
Eag channel Ether-a-go-go Heme Waters q-tof Domain 
ProteomicsCryo-EM structural analysis of FADD: Caspase-8 complexes defines the catalytic dimer architecture for co-ordinated control of cell fate
Uncovering the molecular architecture of the core FADD:Caspase-8 complex and how this is altered by regulatory partners, such as the cell death inhibitor c-FLIP, is essential to understand co-ordination of cell fate. Here, using electron microscopy, we visualize for the first time fulllength proca...
ORGANISM(S): Homo sapiens (Human) 
2021-02-08 | PXD022408 | Pride
Caspase 8 Cell death. disc Fadd Em C-flip complex Structure 
ProteomicsThe HDAC1/DNTTIP1/Flag-tagged MIDEAS (Tyr654Ser) complex was expressed by transient transfection in HEK293F cells. The complex was purified using anti-flag resin and gel filtration. You from here
Analysis of the phosphorylation state of a mis-sense variant in MIDEAS (Tyr654Ser). The substitution from YTP to STP generates a CDK kinase site that is phosphorylated in a human cell line.
ORGANISM(S): Homo sapiens (Human) 
2026-08-18 | PXD064578 | Pride
Human Hdac1 Tdif Histone deacetylase Elmsan1 Dnttip1 Mideas 
ProteomicseIF4A2 drives repression of translation at initiation by Ccr4-Not through purine rich motifs in th 5'UTR
Here we show eIF4A2 is a major effector of the represive miRNA pathway functioning via the Ccr4_Not complex; through its interaction with the Ccr4-Not complex eIF4A2 represses mRNAs at translation initiation. We show evidence that native eIF4A2 has similar properties to chemically inhibited eIF4A1...
ORGANISM(S): Homo sapiens (Human) 
2019-10-10 | PXD015772 | Pride
Translation Eif4a2 Initiation Ddx6 Mrna 
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