Sort   by:  
 Page size 
ProteomicsRevealing the human cancer-associated mucinome
Mucin domains are densely O-glycosylated modular protein domains found in a wide variety of cell surface and secreted proteins. Mucin-domain glycoproteins are key players in a host of human diseases, especially cancer, but the scope of the mucinome remains poorly defined. Recently, we characterized ...
ORGANISM(S): Homo sapiens (Human) 
2022-06-29 | PXD024995 | Pride
Mucin Cancer Mucinase Proteomics Mucinome Mass spectrometry Glycoproteomics O-glycosylation 
ProteomicsOn-tissue spatially-resolved glycoproteomics guided by N-glycan imaging reveal global dysregulation of canine glioma glycoproteomic landscape
Here we present an approach to identify N-linked glycoproteins and deduce their spatial localization using a combination of MALDI N-glycan MSI and spatially-resolved glycoproteomics. We subjected glioma biopsies to on-tissue PNGaseF digestion and MALDI-MSI and found that the glycan HexNAc4-Hex5-NeuA...
ORGANISM(S): Canis familiaris (Dog) (Canis lupus familiaris) 
2022-05-04 | PXD025537 | Pride
Glioblastoma Spatially resolved glycoproteomics Glycoproteomics Glycan imaging Haptoglobin Sialic acid Spatially resolved glycoproteomicsglycan imaging Maldi-msi Hypersialylation 
ProteomicsScreening of the transcriptome, mucinome, and O‐/N‐glycans of the intestinal goblet cell model HT29‐MTX during zinc deficiency
Zinc deficiency is a global health problem affecting approximately one billion people and is associated with serious health consequences, including impaired immune function and an increased susceptibility to infections and inflammation. It is also linked to disrupted intestinal homeostasis and a hig...
ORGANISM(S): Homo sapiens (Human) 
2026-09-14 | PXD080424 | Pride
Glycosylation Mucinome Goblet cell Zinc deficiency 
ProteomicsEye opening discoveries: uncovering lacritin glycoforms and the tear fluid glycoproteome
Tear fluid comprises a diverse group of extracellular glycoproteins which are critical for ocular homeostasis. Within the tear fluid glycoproteome, lacritin is highly expressed and plays a key role in immune response, tear secretion, and antimicrobial activity. Importantly, glycosylation constitutes...
ORGANISM(S): Homo sapiens (Human) 
2025-08-25 | PXD064788 | Pride
Glycoproteomics Mucin Mucinase Lacritin Mass spectrometry Immunoglobulin a Tear fluid 
ProteomicsGlycoFASP: A Universal Method to Prepare Complex Mixtures for O-Glycoproteomic Analysis
Here we describe a method titled filter-aided preparation of O-glycoproteomic samples (GlycoFASP) which takes advantage of molecular-weight cut-off filters and O-glycoprotease digestion specificity. Using GlycoFASP, preparation for MS analysis of biological samples is completed within one day and on...
ORGANISM(S): Homo sapiens (Human) 
2026-07-08 | PXD061804 | Pride
Human Method Serum Lc-ms Glycoproteomics O-glycoproteomics Seminal fluid 
ProteomicsComprehensive analysis of platelet glycoprotein Ibα glycosylation.
Background: Platelet glycoprotein (GP) Ibα is the major ligand-binding subunit of the GPIb-IX-V complex that binds von Willebrand Factor (VWF). GPIbα is heavily glycosylated, and its glycans have been proposed to play key roles in platelet clearance, VWF binding, and as target antigens in immune thr...
ORGANISM(S): Homo sapiens (Human) 
2023-02-09 | PXD035030 | Pride
Human Platelets Glycoproteins 
ProteomicsQuantification and site-specific analysis of co-occupied N- and O-glycopeptides
Protein glycosylation is a complex post-translational modification that is generally classified as N- or O-linked. Site-specific analysis of glycopeptides is accomplished with a variety of fragmentation methods, depending on the type of glycosylation being investigated and the instrumentation availa...
ORGANISM(S): Homo sapiens (Human) 
2025-04-08 | PXD062628 | Pride
Mucin Glycoproteomics O-glycosylation Glycosylation N-glycosylation 
ProteomicsGlycoproteoforms of osteoarthritis-associated lubricin in plasma and synovial fluid
Lubricin or proteoglycan-4 (PRG-4) is a mucinous glycoprotein that lubricates the cartilage and maintains normal tissue function and cell homeostasis. Altered O- glycoproteforms of lubricin have been found in osteoarthritis (OA) synovial fluid (SF), which could ostensibly be used as a biomarker to...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD055049 | Pride
Human Oa Lc-ms Synovial fluid Plasma Lubricin 
ProteomicsPNGaseF-generated N-glycans adduct onto peptides in the gas phase
Glycoproteomics has recently increased in popularity due to instrumental and methodological advances. That said, O-glycoproteomic analysis is still challenging for various reasons, including signal suppression and splitting, search algorithm limitations, and co-occupancy of N- and O-glycopeptides. T...
ORGANISM(S): Homo sapiens (Human) 
2026-06-29 | PXD055856 | Pride
Adducts Glycoproteomics Mucinomics Pngasef Mass spectrometry N-glycans 
ProteomicsGlycosite Mapping and in situ Mass Spectrometry Imaging of MUC2 Glycopeptides via On-slide Mucinase Digestion
Aberrant mucin expression and glycosylation are hallmarks of cancer, but how these changes promote malignant processes are not well understood. Solid tumors are highly heterogeneous in their cellular and molecular composition, and many advanced spatial techniques have emerged in recent years to stud...
ORGANISM(S): Homo sapiens (Human) 
2026-04-06 | PXD055865 | Pride
Sort   by:  
 Page size