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ProteomicsDefining the T cell proteome and the role of mTORC1 during differentiation using quantitative proteomics
High-resolution quantitative mass-spectrometry reveals similarities and disparities in how murine naïve CD4 and CD8 T cells respond to immune activation and re-structure proteomes as they differentiate to effector cells. The data map core transcriptional, metabolic and protein synthesis machinery, n...
ORGANISM(S): Mus musculus (Mouse) 
2019-03-28 | PXD012058 | Pride
Mouse T cell Mtorc1 Fusion Tmt Mtor Rapamycin 
ProteomicsThe impact of hypoxia on the cytotoxic T lymphocyte proteome
Metabolic and nutrient-sensing pathways play an important role in controlling the efficacy of effector T cells. Oxygen is a critical regulator of cellular metabolism. However, during immune responses T cells must function in oxygen-deficient, or hypoxic, environments. Here, we used high resolution m...
ORGANISM(S): Mus musculus (Mouse) 
2021-10-20 | PXD026223 | Pride
Cd8 t cells Oxygen sensing Hypoxia Ctls Cytotoxic t lymphocytes 
ProteomicsSuper-killer CTLs are generated by single gene deletion of Bach2
Bach2 codes for a transcriptional regulator exerting major influences on T cell mediated immune regulation. Effector CTLs derived from in vitro activation of murine CD8+ T cells showed increased proliferative and cytolytic capacity in the absence of BACH2. Before activation, BACH2-deficient CD8+ T c...
ORGANISM(S): Mus musculus (Mouse) 
2022-09-02 | PXD036457 | Pride
T cell Proteomics Bach2 Cd8 Ctl Tmt 
ProteomicsNrf2 is a central regulator of the metabolic landscape in macrophages and finetunes their inflammatory response
To overcome oxidative, inflammatory, and metabolic stress, cells have evolved networks of cytoprotective proteins controlled by transcription factor nuclear factor erythroid 2 p45-related factor 2 (Nrf2) and its main negative regulator the Kelch-like ECH associated protein 1 (Keap1). Here, we used h...
ORGANISM(S): Mus musculus (Mouse) 
2021-08-13 | PXD027737 | Pride
Tmt Keap1 Nrf2 Mitochondria Bmdm Murine 
ProteomicsQuantitative phosphoproteomics of cytotoxic T cells to reveal Protein Kinase D 2 regulated networks
The focus of the present study was to characterize the phosphoproteome of cytotoxic T cells and to explore the role of the serine threonine kinase PKD2 (Protein Kinase D2) in the phosphorylation networks of this key lymphocyte population. We used Stable Isotope Labelling of Amino acids in Culture (S...
ORGANISM(S): Mus musculus (Mouse) 
2014-10-03 | PXD001076 | Pride
Silac Pkd2 Phosphoproteomics Lymphocytes 
ProteomicsImpact of Acute methionine restriction on proteome of Th1 cells - Part 2
CD4 T cells were activated using CD3/CD28 antibodies in the presence of IL2 and IL12, to generate Th1 cells. Th1 cells were maintained and expanded in IL2 and IL12, on D5 Th1 cells were sorted for CD4+ expression and DAP1 exclusion. After flow sorting, live TH1 cells were resuspended (1e6 per ml) in...
ORGANISM(S): Mus musculus (Mouse) 
2019-03-29 | PXD012053 | Pride
Metabolism Methioine Transport T cells 
ProteomicsIn-depth analysis of the phosphoprotein landscape of cytotoxic T lymphocytes and its regulation by cytokines
Here we present high-resolution mass spectrometry analysis of changes to the phosphoproteome of cytotoxic T lymphocytes (CTL) induced by the cytokine, Interleukin 12 (IL-12). We treated CTL with a short and long IL-12 stimulation and mapped the changes to the phosphoproteome using quantitative SILAC...
ORGANISM(S): Mus musculus (Mouse) 
2016-08-24 | PXD004644 | Pride
Silac Il-12 Phosphoproteomics Lymphocytes 
ProteomicsPhosphoproteomic analyses of IL-2 signaling reveals integrated JAK-dependent and independent phosphorylation networks that drive cytotoxic T cell fate
High-resolution mass spectrometry analysis of Interleukin 2 (IL-2) and Janus kinase (JAK) controlled protein phosphorylations in cytotoxic T lymphocytes (CTL) revealed JAKs coupled IL-2 receptors to diverse and complex serine/threonine kinase-substrate networks. These involved intricate, co-ordinate...
ORGANISM(S): Mus musculus (Mouse) 
2016-08-25 | PXD004645 | Pride
Lck/fyn Lymphocytes. Silac Jak1 Il-2 Jak3 Phosphoproteomics 
ProteomicsConcomitant deletion of Shp-1 and Shp-2 in T cells fails to improve anticancer responses 
Anticancer T cells acquire a dysfunctional state characterized by poor effector function and expression of inhibitory receptors, such as programmed cell death protein 1 (PD-1). Blockade of PD-1 signalling leads to T cell reinvigoration and is increasingly applied as an effective anticancer treatment...
ORGANISM(S): Mus musculus (Mouse) 
2022-09-29 | PXD034897 | Pride
Shp-2  shp-1  ptpn11  ptpn6  t cell exhaustion  cancer immunotherapy  inhibitory receptors  pd-1 checkpoint therapy  
ProteomicsControl of amino-acid transport coordinates metabolic reprogramming in T cell malignancy
Analysis of murine model of T cell acute lymphoblastic leukemia (T-ALL) caused by deletion of phosphatase and tensin homologue deleted on chromosome 10 (PTEN) reveals that primary T-ALL cells have high transport rates for multiple nutrients. In particular, high levels of leucine transport in T-ALL f...
ORGANISM(S): Mus musculus (Mouse) 
2018-10-08 | PXD006209 | Pride
Pten T-all Hif T cells 
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