Sort   by:  
 Page size 
ProteomicsYeast exosome: immunoprecipitation 2
Nuclear RNA degradation pathways are highly conserved across eukaryotes and play important roles in RNA quality control. Key substrates for exosomal degradation include aberrant functional RNAs and cryptic unstable transcripts (CUTs). It has recently been reported that the nuclear exosome is inactiv...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2015-05-15 | PXD001239 | Pride
Exosome Meiosis Yeast 
ProteomicsYeast exosome: immunoprecipitation 1
Nuclear RNA degradation pathways are highly conserved across eukaryotes and play important roles in RNA quality control. Key substrates for exosomal degradation include aberrant functional RNAs and cryptic unstable transcripts (CUTs). It has recently been reported that the nuclear exosome is inactiv...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2015-05-15 | PXD001223 | Pride
Exosome Meiosis Yeast 
ProteomicsFgf and Esrrb integrate epigenetic and transcriptional networks that regulate self-renewal of Trophoblast Stem Cells
Esrrb is a transcription factor implicated in embryonic stem (ES) cell self-renewal, yet its knockout causes intrauterine lethality due to defects in trophoblast development. Here we show that in trophoblast stem (TS) cells, Esrrb is a downstream target of fibroblast growth factor (Fgf) signalling a...
ORGANISM(S): Mus musculus (Mouse) 
2015-07-24 | PXD002183 | Pride
Ts cells Esrrb Self-renewal Transcription factors Lc-ms/ms 
ProteomicsModified cytosine binding proteins
DNA methylation (5mC) plays important roles in epigenetic regulation of genome function, and recently the TET1-3 hydroxylases have been found to oxidize 5mC to hydroxymethylcytosine (5hmC), formylcytosine (5fC), and carboxylcytosine (5caC) in DNA. These derivatives have a role in demethylation of DN...
ORGANISM(S): Mus musculus (Mouse) 
2013-10-21 | PXD000524 | Pride
Formylcytosine Pax6 Methylcytosine Hydroxymethylcytosine Es cells Fgf15 
ProteomicsA cyclic peptide toolkit reveals mechanistic principles of peptidylarginine deiminase IV (PADI4) regulation
Deregulation of the peptidylarginine deiminase PADI4 promotes the development of autoimmunity, cancer, atherosclerosis and age-related tissue fibrosis. Genetic or pharmacological PADI4 inhibition is therapeutically efficacious in mice but no clinically relevant inhibitors exist to date. PADI4 additi...
ORGANISM(S): Mus musculus (Mouse) 
2025-05-06 | PXD048807 | Pride
Deiminase Cyclic-peptide Modulator Padi4 
ProteomicsThe Rac-GEF Tiam1 controls integrin-dependent neutrophil responses
Rac GTPases are required for neutrophil adhesion and migration, and for the neutrophil effector responses that kill pathogens. These Rac-dependent functions are impaired when neutrophils lack the activators of Rac, Rac-GEFs from the Prex, Vav and Dock families. In this study, we demonstrate that Tia...
ORGANISM(S): Mus musculus (Mouse) 
2024-01-26 | PXD046791 | Pride
Tiam1 Dock2 Vav Neutrophils Rac Prex1 Rac-gef Small gtpase 
ProteomicsPLEKHS1 drives PI3Ks and remodels pathway homeostasis in PTEN-null prostate
The PIP3/PI3K network is a central regulator of metabolism and is frequently activated in cancer, commonly by loss of the PIP3/PI(3,4)P2-phosphatase, PTEN. Despite huge investment, the drivers of the PI3K network in normal tissues and how they adapt to overactivation are unclear. We find that in hea...
ORGANISM(S): Mus musculus (Mouse) 
2023-08-23 | PXD043239 | Pride
Irs1 Pten Plekhs1 Prostate Pi3k 
ProteomicsAn Nlrp5-null mutation leads to attenuated de novo methylation in oocytes, accompanied by significant reduction in DNMT3L
Nlrp5 encodes a core component of the subcortical maternal complex (SCMC) a cytoplasmic protein structure unique to the mammalian oocyte and cleavage-stage embryo. NLRP5 mutations have been identified in patients presenting with early embryo arrest, recurrent molar pregnancies and imprinting disorde...
ORGANISM(S): Mus musculus (Mouse) 
2025-12-22 | PXD056859 | Pride
Nlrp5 Subcortical maternal complex Oocyte Dna methylation 
GenomicsGenome-wide distribution of 5-formylcytosine in ES cells is associated with transcription and depends on TDG
Methylation of cytosine in DNA (5mC) is an important epigenetic mark that is involved in the regulation of genome function. During early embryonic development in mammals, the DNA methylation landscape is dynamically reprogrammed in part through active demethylation. Recent advances have identified k...
ORGANISM(S): Mus musculus 
2012-08-17 | E-GEOD-40148 | biostudies-arrayexpress
ProteomicsSolvent Precipitation SP3 (SP4) enhances recovery for proteomics sample preparation without magnetic beads
Complete, reproducible extraction of protein material is essential for comprehensive and unbiased proteome analyses. A current gold standard is single-pot, solid-phase-enhanced sample preparation (SP3), in which organic solvent and magnetic beads are used to denature and capture protein aggregates, ...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) Drosophila melanogaster (Fruit fly) 
2022-08-01 | PXD032095 | Pride
Microparticles Proteomics Sample preparation Protein aggregation Contaminant removal Lc−ms/ms Protein precipitation 
Sort   by:  
 Page size