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ProteomicsMonitor precursor mass shifts of Halobacterium salinarum
Approximately 2x106 CL1-5 and H23 cells were seeded on 10-cm dishes with RPMI-1640 medium containing 10% fetal bovine serum. After the cells covered 80% of the dish, cells were serum-starved for 24 h in RPMI-1640 medium. CL1-5 and H23 cell lysates were harvested, trypsinized and analyzed by using LT...
ORGANISM(S): Homo sapiens (Human) Halobacterium salinarum 
2020-01-14 | PXD000082 | Pride
Cl1-5 lysate Nrc-1 gvd lysate Nrc-1 lysate Ltq-orbitrap xl Ltq-orbitrap discovery H23 lysate Human nsclc cell line Halobacterium salinarum nrc-1 Lc-ms/ms 
ProteomicsRat Kir4.1 interactome with the particular purpose to identify 10 kDa copurified proteins using LC-MSMS
The project aims to determine the interactome of full-length Kir4.1 overexpressed in HEK293 stable cells. The overexpressed proteins were purified, and the purified samples, along with three bands from a 1D SDS-PAGE gel, were analyzed by LC-MSMS after trypsin digestion. The primary goal was to ident...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD060340 | Pride
Lc-msms Hek293 stable cells Full length rat kir4.1 Lc8 copurification 
ProteomicsMouse Muscle Quadricep Methylation CARM1 KO
CARM1 muscle KO mice were generated and quadricep muscle tissue was subjected to high pH strong cation exchange and immunoaffinity purification to enrich methyl peptides.
ORGANISM(S): Mus musculus (Mouse) 
2022-08-05 | PXD028884 | Pride
Arginine Carm1 Prmt4 Immunoaffinity Adma Methylation Mouse Sdma Lcms Muscle 
ProteomicsA conserved long intergenic non-coding RNA containing snoRNA sequences, lncCOBRA1, affects Arabidopsis germination and development
Long non-coding RNAs (lncRNAs) are an increasingly studied group of non-protein coding transcripts with a wide variety of molecular functions gaining attention for their roles in numerous biological processes. Nearly 6,000 lncRNAs have been identified in Arabidopsis thaliana but many have yet to be ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-08-12 | PXD033707 | Pride
Lincrna  snorna  rbp  chirp  arabidopsis  development 
ProteomicsProximal proteomics connects NRF2 suppression to ZNF746/PARIS-driven oxidative stress and apoptosis in Parkinson’s disease
The nuclear factor erythroid 2-related factor 2 (NRF2) transcription factor activates cytoprotective and metabolic gene expression in response to various electrophilic stressors. In disease, constitutive NRF2 activity promotes cancer progression while decreased NRF2 function contributes to neurodege...
ORGANISM(S): Homo sapiens (Human) 
2023-12-18 | PXD043182 | Pride
Znf746 Proximity proteomics Mafg Nrf2 Nrf3 Nrf1 Parkinson's disease Oxidative stress 
ProteomicsMAFG proximity proteomic analysis in melanoma cells
Proximity proteins of melanoma cell line WM164 expressing TurboID-NLS or TurboID-MAFG was analyzed.
ORGANISM(S): Homo sapiens (Human) 
2026-02-19 | PXD055213 | Pride
Turboid Mafg Proximity Melanoma 
ProteomicsYield and immunomodulatory effects of pneumococcal membrane vesicles change with bacterial growth phase
We investigated whether isolation of MVs during either the stationary-phase (24 h) or death-phase (48 h) affected their yield, immunogenicity and cytotoxicity in primary human immune cells. Death-phase vesicles showed higher yield than stationary-phase vesicles. Both vesicle types displayed excellen...
ORGANISM(S): Streptococcus pneumoniae (strain ATCC BAA-255 / R6) 
2022-02-16 | PXD026462 | Pride
Proteomics Primary macrophages Death-phase Stationary-phase Bacterial membrane vesicles Vaccine Immunogenicity 
ProteomicsCompositional analysis of extracellular aggregates in the eyes of patients with exfoliation syndrome and exfoliation glaucoma.
Exfoliation syndrome (XFS) is a condition characterized by the production of insoluble fibrillar aggregates (exfoliation material; XFM) in the eye and elsewhere. Many patients with XFS progress to exfoliation glaucoma (XFG), a significant cause of global blindness. We used quantitative mass spectrom...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD026764 | Pride
Human Exfoliation syndrome Shotgun proteomics Psr-ohsu Lens capsule Aqueous humor Tmt labeling Quantitative proteomics Exfoliation glaucoma 
ProteomicsEvaluation of fully functionalized diazirine tags for chemical proteomic applications
The use of photo-affinity labeling (PAL) reagents for the mapping of noncovalent small molecule-protein interactions has become widespread. Recently, several ‘fully-functionalized’ (FF) chemical tags have been developed wherein a photoactivatable capture group, an enrichment handle, and a functional...
ORGANISM(S): Homo sapiens (Human) 
2021-05-18 | PXD025652 | Pride
Fully-functionlalized Diazirine Photoaffinity 
ProteomicsEpitope Mapping of Human Polyclonal Antibodies to the fHbp Antigen of a Neisseria Meningitidis Vaccine by Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) - part 2
Antigen-antibody interactions play a key role in the immune response post vaccination and the mechanism of action of antibody-based biopharmaceuticals. 4CMenB is a multicomponent vaccine against Neisseria meningitidis serogroup B in which factor H binding protein (fHbp) is one of the key antigens. I...
ORGANISM(S): Homo sapiens (Human) 
2024-05-22 | PXD047712 | Pride
Pab Fhbp Hdx-ms 
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